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KMID : 1094719970020010023
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 1 p.23 ~ p.26
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Strain development for the production of D(?)-β-hydroxyisobutyric acid
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Seong Dong-Ho
Paik Woo-Hyun
Kim Nam-Ki
Baek Seung-Woo
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Abstract
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Candida rugosa BR-120, which cannot catabolize D(?)-β-hydroxyisobutyric acid (D-HIBA), was developed fromCandida rugosa IFO 0750 by UV irradiationC. rugosa BR-120 could not assimilate propionic acid as a carbon source. It is presumed thatC. rugosa BR-120 lacks HIBA dehydrogenase.C. rugosa BR-120 produced D-HIBA from isobutyric acid (IBA) with a high yield about 94%, whileC. rugosa IFO 0750 did it with about 42% on 5 days batch cultivation. On 5 days fed-batch cultivation,C. rugosa BR-120 andC. rugosa IFO 0750 produced 12.45 g/L and 5.22 g/L of D-HIBA, respectively. The degradation rate of D-HIBA by the resting cell ofC. rugosa IFO 0750 was 31.95%, butC. rugosa BR-120 was 3.08% on 2 days incubation.
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KEYWORD
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D(?)-β-hydroxyisobutyric acid, isobutyric acid, Candida rugosa
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